Evidence for early stages of somatic embryo development in a protoplast‐derived cell culture of Prunus avium
Identifieur interne : 003996 ( Main/Exploration ); précédent : 003995; suivant : 003997Evidence for early stages of somatic embryo development in a protoplast‐derived cell culture of Prunus avium
Auteurs : Hélène David [France] ; Jean-Marc Domon [France] ; Colette Savy [France] ; Nicole Miannay [France] ; Gérard Sulmont [France] ; Robert Dargent [France] ; Alain David [France]Source :
- Physiologia Plantarum [ 0031-9317 ] ; 1992-06.
English descriptors
- KwdEn :
Abstract
In the genus Prunus, so far, somatic embryogenesis has not been reported either from cell suspensions or from their protoplast‐derived cells. Rhizogenic cell suspensions of Prunus avium L., initiated from adventitious roots developed from cotyledon‐derived callus of mature zygotic embryos, have been subcultured for more than one year without losing their morphogenic potential. A yield of 8 × 105 protoplasts ml−1 of packed cells with a viability of 98% has been routinely obtained. Optimum cell division frequency (around 2.5% at day 10 and 4–6% at day 15) occurs in agarose lenses, with Murashige and Skoog (1962. Physiol. Plant 15: 476–497)‐based medium supplemented with 5 μM naphthalene acetic acid, 1 μM benzyladenine and 0.25 μM zeatin. Colony formation has been achieved after 35 days with a plating efficiency of 3–4%. Cell suspensions have been initiated from protoplast‐derived callus. While the older cell cultures express a rhizogenic response, the younger ones contain early stages of somatic embryo development. Ultrastructural examination confirms the polarization of these structures.
Url:
DOI: 10.1111/j.1399-3054.1992.tb04738.x
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000460
- to stream Istex, to step Curation: 000460
- to stream Istex, to step Checkpoint: 001153
- to stream Main, to step Merge: 003D04
- to stream Main, to step Curation: 003996
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Evidence for early stages of somatic embryo development in a protoplast‐derived cell culture of Prunus avium</title><author><name sortKey="David, Helene" sort="David, Helene" uniqKey="David H" first="Hélène" last="David">Hélène David</name></author><author><name sortKey="Domon, Jean Arc" sort="Domon, Jean Arc" uniqKey="Domon J" first="Jean-Marc" last="Domon">Jean-Marc Domon</name></author><author><name sortKey="Savy, Colette" sort="Savy, Colette" uniqKey="Savy C" first="Colette" last="Savy">Colette Savy</name></author><author><name sortKey="Miannay, Nicole" sort="Miannay, Nicole" uniqKey="Miannay N" first="Nicole" last="Miannay">Nicole Miannay</name></author><author><name sortKey="Sulmont, Gerard" sort="Sulmont, Gerard" uniqKey="Sulmont G" first="Gérard" last="Sulmont">Gérard Sulmont</name></author><author><name sortKey="Dargent, Robert" sort="Dargent, Robert" uniqKey="Dargent R" first="Robert" last="Dargent">Robert Dargent</name></author><author><name sortKey="David, Alain" sort="David, Alain" uniqKey="David A" first="Alain" last="David">Alain David</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:D7B8863CA8D9BB469FA5A6358FE90DEEA8A8AF04</idno><date when="1992" year="1992">1992</date><idno type="doi">10.1111/j.1399-3054.1992.tb04738.x</idno><idno type="url">https://api.istex.fr/document/D7B8863CA8D9BB469FA5A6358FE90DEEA8A8AF04/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000460</idno><idno type="wicri:Area/Istex/Curation">000460</idno><idno type="wicri:Area/Istex/Checkpoint">001153</idno><idno type="wicri:doubleKey">0031-9317:1992:David H:evidence:for:early</idno><idno type="wicri:Area/Main/Merge">003D04</idno><idno type="wicri:Area/Main/Curation">003996</idno><idno type="wicri:Area/Main/Exploration">003996</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Evidence for early stages of somatic embryo development in a protoplast‐derived cell culture of Prunus avium</title><author><name sortKey="David, Helene" sort="David, Helene" uniqKey="David H" first="Hélène" last="David">Hélène David</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Lab. de Biotechnologies Végétales, Univ. de Picardie, 33, rue Saint‐Leu, F‐80039 Amiens</wicri:regionArea><placeName><region type="region" nuts="2">Hauts-de-France</region><region type="old region" nuts="2">Picardie</region><settlement type="city">Amiens</settlement></placeName></affiliation></author><author><name sortKey="Domon, Jean Arc" sort="Domon, Jean Arc" uniqKey="Domon J" first="Jean-Marc" last="Domon">Jean-Marc Domon</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Lab. de Biotechnologies Végétales, Univ. de Picardie, 33, rue Saint‐Leu, F‐80039 Amiens</wicri:regionArea><placeName><region type="region" nuts="2">Hauts-de-France</region><region type="old region" nuts="2">Picardie</region><settlement type="city">Amiens</settlement></placeName></affiliation></author><author><name sortKey="Savy, Colette" sort="Savy, Colette" uniqKey="Savy C" first="Colette" last="Savy">Colette Savy</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Lab. de Cryptogamie, Différenciation fongique, Univ. Paul Sabatier, 118, route de Narbonne, F‐31062 Toulouse</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Midi-Pyrénées</region><settlement type="city">Toulouse</settlement></placeName></affiliation></author><author><name sortKey="Miannay, Nicole" sort="Miannay, Nicole" uniqKey="Miannay N" first="Nicole" last="Miannay">Nicole Miannay</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Lab. de Biotechnologies Végétales, Univ. de Picardie, 33, rue Saint‐Leu, F‐80039 Amiens</wicri:regionArea><placeName><region type="region" nuts="2">Hauts-de-France</region><region type="old region" nuts="2">Picardie</region><settlement type="city">Amiens</settlement></placeName></affiliation></author><author><name sortKey="Sulmont, Gerard" sort="Sulmont, Gerard" uniqKey="Sulmont G" first="Gérard" last="Sulmont">Gérard Sulmont</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Lab. de Biotechnologies Végétales, Univ. de Picardie, 33, rue Saint‐Leu, F‐80039 Amiens</wicri:regionArea><placeName><region type="region" nuts="2">Hauts-de-France</region><region type="old region" nuts="2">Picardie</region><settlement type="city">Amiens</settlement></placeName></affiliation></author><author><name sortKey="Dargent, Robert" sort="Dargent, Robert" uniqKey="Dargent R" first="Robert" last="Dargent">Robert Dargent</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Lab. de Cryptogamie, Différenciation fongique, Univ. Paul Sabatier, 118, route de Narbonne, F‐31062 Toulouse</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Midi-Pyrénées</region><settlement type="city">Toulouse</settlement></placeName></affiliation></author><author><name sortKey="David, Alain" sort="David, Alain" uniqKey="David A" first="Alain" last="David">Alain David</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Lab. de Biotechnologies Végétales, Univ. de Picardie, 33, rue Saint‐Leu, F‐80039 Amiens</wicri:regionArea><placeName><region type="region" nuts="2">Hauts-de-France</region><region type="old region" nuts="2">Picardie</region><settlement type="city">Amiens</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Physiologia Plantarum</title><idno type="ISSN">0031-9317</idno><idno type="eISSN">1399-3054</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1992-06">1992-06</date><biblScope unit="volume">85</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="301">301</biblScope><biblScope unit="page" to="307">307</biblScope></imprint><idno type="ISSN">0031-9317</idno></series><idno type="istex">D7B8863CA8D9BB469FA5A6358FE90DEEA8A8AF04</idno><idno type="DOI">10.1111/j.1399-3054.1992.tb04738.x</idno><idno type="ArticleID">PPL301</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0031-9317</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Protoplast</term><term>Prunus avium</term><term>somatic embryogenesis</term><term>woody plant</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In the genus Prunus, so far, somatic embryogenesis has not been reported either from cell suspensions or from their protoplast‐derived cells. Rhizogenic cell suspensions of Prunus avium L., initiated from adventitious roots developed from cotyledon‐derived callus of mature zygotic embryos, have been subcultured for more than one year without losing their morphogenic potential. A yield of 8 × 105 protoplasts ml−1 of packed cells with a viability of 98% has been routinely obtained. Optimum cell division frequency (around 2.5% at day 10 and 4–6% at day 15) occurs in agarose lenses, with Murashige and Skoog (1962. Physiol. Plant 15: 476–497)‐based medium supplemented with 5 μM naphthalene acetic acid, 1 μM benzyladenine and 0.25 μM zeatin. Colony formation has been achieved after 35 days with a plating efficiency of 3–4%. Cell suspensions have been initiated from protoplast‐derived callus. While the older cell cultures express a rhizogenic response, the younger ones contain early stages of somatic embryo development. Ultrastructural examination confirms the polarization of these structures.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Hauts-de-France</li><li>Midi-Pyrénées</li><li>Occitanie (région administrative)</li><li>Picardie</li></region><settlement><li>Amiens</li><li>Toulouse</li></settlement></list><tree><country name="France"><region name="Hauts-de-France"><name sortKey="David, Helene" sort="David, Helene" uniqKey="David H" first="Hélène" last="David">Hélène David</name></region><name sortKey="Dargent, Robert" sort="Dargent, Robert" uniqKey="Dargent R" first="Robert" last="Dargent">Robert Dargent</name><name sortKey="David, Alain" sort="David, Alain" uniqKey="David A" first="Alain" last="David">Alain David</name><name sortKey="Domon, Jean Arc" sort="Domon, Jean Arc" uniqKey="Domon J" first="Jean-Marc" last="Domon">Jean-Marc Domon</name><name sortKey="Miannay, Nicole" sort="Miannay, Nicole" uniqKey="Miannay N" first="Nicole" last="Miannay">Nicole Miannay</name><name sortKey="Savy, Colette" sort="Savy, Colette" uniqKey="Savy C" first="Colette" last="Savy">Colette Savy</name><name sortKey="Sulmont, Gerard" sort="Sulmont, Gerard" uniqKey="Sulmont G" first="Gérard" last="Sulmont">Gérard Sulmont</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003996 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003996 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:D7B8863CA8D9BB469FA5A6358FE90DEEA8A8AF04
|texte= Evidence for early stages of somatic embryo development in a protoplast‐derived cell culture of Prunus avium
}}
| This area was generated with Dilib version V0.6.25. |  |